Washington University is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, age, sex, sexual orientation, gender identity or expression, national origin, genetic information, disability, or protected veteran status.
The hiring range for this position is commensurate with experience.
The Electron Microscopy Specialist works with other EM staff under the supervision of the Director in operation of the electron microscopy component of the Center for Cellular Imaging (http://wucci.wustl.edu/).
PRIMARY DUTIES AND RESPONSIBILITIES:
1. User Training - train users in sample preparation and imaging techniques for electron microscopy.
Provide advanced level instruction and work direction to University laboratory staff on Center instrumentation and procedures used in the various electron microscopy methods supported by the Center.
Train facility users in the appropriate use of data analysis packages and methods, micrograph production, and quantification of data.
Train laboratory staff on different experimental techniques used in the Center
Develop reference tools for lab staff including handouts.
Provide advanced level instruction on an individual level with undergraduate students, graduate students, post-docs, and collaborators on Center equipment and procedures for electron microscopes (e.g. alignment of scope, specimen preparation), ultramicrotomes, computer-aided serial sectioning and reconstruction programs.
2. EM Service - Prepare samples for TEM and SEM and take and analyze images for University researchers.
Work with researchers on the design, preparation, execution and analysis of electron microscopy experiments for both TEM, SEM, cryo-TEM techniques.
Acquire and process biological specimens using perfusion, tissue extraction, sectioning, and chemical fixation techniques.
Carry out immunocytochemical labeling of tissues and cell cultures using monoclonal and polyclonal antibodies by pre-embedding and post-embedding methods.
Prepare specimens using cryogenic techniques such as slam freezing and freeze fracture for the preservation of cellular ultrastructure and membrane morphology.
Keep up with technical advances in specimen preparation by reading primary literature and microscopy web sites.
Reconstruct electron microscopy data sets (serial sections, tomograms etc).
Responsible for basic image analysis, e.g. image averaging, filtering, alignment, quantitative analysis.
3. Research Design & Participation - design experiments and protocols and independently conduct experiments that involve both advanced Transmission and Scanning EM (TEM and SEM) methods such as tomographic imaging, cryo-TEM. Specialist will also assist with image processing and data analysis.
Design, and carry out experiments involving collection and processing of tissues and tissue culture cells and their analysis by electron microscopy.
Provide innovative contributions to the design and construction of experiments for multiple research projects, obtain data, and analyze light and electron micrographs.
Perform all applicable basic and advanced techniques in electron microscopy including transmission EM, chemical fixation and staining, ultracryomicrotomy, and carbon and metal coating of films and specimens at high level of expertise.
Modify and perform experiments involving immunoperoxidase and immunogold localization of specific proteins.
Participate in the collection and analysis of images and application of protocols for quantification of data.
Keep informed of new developments in electron microscopy in the fields of molecular and cell biology.
Collect micrographs and other data for journal articles using photographic and digital imaging procedures.
4. EM Equipment Maintenance - maintenance of the TEM, SEM and sample preparation equipment
Maintain stocks of reagents for methods involving tissue culture, antibody reagents, buffers, etc.
Maintain a monthly freezer inventory of primary and secondary antibodies, seras, toxins and dyes. Defrost and keep general used antibodies stocked. Aliquot new antibodies assign freezer rack and box; enter new antibodies into the database.
Keep database of scientific Center records.
Perform general maintenance of facilities electron microscopes and sample preparation equipment.
Liaise with manufacturers with regard to instrument preventative maintenance.
Ensure assigned areas, and various pieces of equipment are clean and prepared.
Keep a log sheet for controlled substances, check usage of controlled substances regularly and order supplies as needed.
Use scheduling software to prepare monthly invoices for facility user recharges and track of equipment usage to prepare monthly status reports for Director.
Maintain records of equipment usage and report maintenance issues of equipment.
Ensure Center is in compliance with EH&S protocols and procedures.
5. Data collection and presentation - collect and manage data collected on EM instruments, help to prepare data for publication and presentation.
Prepare data for publication; grant applications, posters and other presentations using digital facilities.
Present data at department or University meetings.
Prepare images/movies for presentations.
Assist in preparation of manuscripts, grants, progress reports and annual protocols.
Prepare publication quality micrographs and plates.
Maintain lab notebook, including experimental design, time, methods, materials and results.
Master’s degree in a related field with 7 years of experience in a laboratory setting or equivalent combination of relevant education and work experience equaling at least 10 years.
This position is full-time and works approximately 40 hours per week.
Department Name/Job Location:
This position at the Center for Cellular Imaging is affiliated with the Departments of Neuroscience and Cell Biology & Physiology. This position is for the Medical School Campus.
Demonstrated skill and experience in the high-resolution operation of electron microscopes including scanning and transmission methods.
Theoretical and practical knowledge of biology or related scientific field and of tissue structure on an anatomical, cellular, and molecular level.
Understanding both the theory and practice of various immunochemical localization labeling techniques for scanning and transmission electron microscopy.
Working knowledge of immunofluorescence, immunoperidoxidase and immunogold labeling methods on semi- and ultrathin tissue sections and on tissue culture cells.
Experience in performing experimental protocols unsupervised and in recommending modifications to procedures and protocols to meet the needs of research projects.
Ability to contribute to experimental design and assist with the analysis and interpretation of results.
Proven organizational and time management skills to successfully set priorities, meet established deadlines and recognize new problems with constantly changing priorities and frequent interruptions.
Strong communication skills (both verbal and written) needed to interact professionally and effectively in the work environment. Ability to read, comprehend, and discuss research materials. Proven ability to write, edit and proofread research results. Skill at explaining difficult concepts and training users and students.
Ability to diplomatically and professionally interact with all levels of University faculty and staff and external contacts.
Proven ability to perform minor maintenance and alignment of electron microscopes.
Comprehensive knowledge of photographic techniques including visual arts production, copy work, photographic printing and enlarging, and slide making.
This position is eligible for full-time benefits.� Please visit our website at http://hr.wustl.edu to view a summary of benefits.
Internal Applicant Instruction:
This position is grade R09.
Please attach a copy of your most current signed performance evaluation (completed within the last 18 months) to your online account. If you have not received a performance evaluation, you may provide two current signed letters of recommendation (written within the last 18 months), preferably to include one letter from either a current or recent former supervisor. To attach these documents, go to: My Career Tools, Add Attachment, Attachment Type – Performance Reviews or Letters of Recommendation.
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