Duties/Responsibilities: We are seeking a highly self-motivated and organized individual for a Researcher 1 position to join our group of researchers studying heart and muscle regeneration. The research of the laboratory focuses on identifying genes that are involved in the development and regeneration processes. This individual will work closely with a senior member of the laboratory and assist with the experiments and animal husbandry.
40% Molecular biology
Design primers for PCR (polymerase chain reaction) and run PCR, purify DNA and RNA, construct plasmid vectors, prepare plasmids with miniprep and midiprep and analyze DNA sequences.
40% Cell biology
Culture of stem cells, induce differentiation of stem cells, plasmid transfection.
20% General lab maintenance
Make solutions and gels for electrophoresis, organize reagents and chemicals, write and organize lab manuals and other lab maintenance jobs as needed.
All required qualifications must be documented on application materials.
Bachelor's degree in biology or a related field of science or a combination of related education and work experience to equal four years.
Experience in molecular biology techniques.
Documented basic knowledge in genetics, biochemistry, and molecular biology with related course works.
2 years of research lab experience in molecular biology techniques.
The ability/experience designing and constructing plasmid vectors and analyzing DNA sequences with web-based programs (BLAST and Pubmed).
Proven ability to meticulously and accurately record experiments in lab notebooks.
Experience in molecular biology techniques, PCR, cell/tissue culture, mouse husbandry, sectioning for histology, immunohistochemistry, biochemical analysis of protein expression.
Experience of gene editing using the CRISPR/Cas9 technology will be given high preference.
The University of Minnesota, founded in the belief that all people are enriched by understanding, is dedicated to the advancement of learning and the search for truth; to the sharing of this knowledge through education for a diverse community; and to the application of this knowledge to benefit the people of the state, the nation, and the world.