Research in the laboratory focuses on understanding the biological principles that govern the pathogenesis of insulin-producing cells, implicated in both type1 and type 2 diabetes. The lab is invested in understanding the role of the fetal environment in the womb in the regulation of basal beta-cell mass and function. The lab uses genetic mouse models to study the function of nutrient sensor proteins mTOR and OGT in utero and in adult mice. The long term goal is to translate these fundamental biologic principles into clinically useful therapeutic strategies and diagnostic tools.
In this position the candidate will perform cell culture and islet isolation. Responsible for maintaining an inventory of frozen cell stocks, virus, plasmid. Will isolate or provide assistance to lab personnel with islet isolation. Process islets by extracting DNA and RNA, as well as perform cloning and subcloning of DNA fragments. Independently extract DNA and RNA from samples for genotyping or qpcr. Perform molecular biology/cell biologic assays, to study mouse models of diabetes and fetal programming of obesity/diabetes. With assistance from Researcher 1, he/she will maintain the animal colony of the lab.
They will act as the Area Safety Coordinator for assigned laboratory space. Responsible for EH&S compliance of the laboratory and the required training of lab personnel. Preparation of chemical, biological, and radiation protocols for submission to EH&S committees for review. Assist Principle Investigator with personnel matters. Will integrate information obtained from scientific journals for use in the laboratory and disseminate this information to other lab members.
Responsibilities also include: preparing media and reagents, maintaining laboratory organization and laboratory supplies, purchasing laboratory supplies, and other critical functions as needed for the laboratory to run smoothly.
1) Cell biology (30%)
a. Routine tissue culture (cell lines and primary islets). b. Transfection of plasmids, siRNA, or other genetic material into tumor cell lines c. Perform Florescent Activated Cell Sorting (FACS) experiments via the CCRB Core, and process samples and prepare data derived for analysis. d. Islet Isolation (will be trained) e. Perform Western blotting and ELISA experiments f. Perform cellular fractionation experiments g. Pulse-chase experiment (will be trained).
2) Molecular biology (30%)
a. Extract DNA and RNA from islets or embryonic beta-cells, or placenta. b. Preparation of cDNA from RNA c. Perform PCR and qPCR reactions d. Perform cloning and subcloning of pertinent DNA fragments e. DNA binding assays, ChIP Assays, and prepare samples for ChIP seq assays
3) Animal studies (30%)
a. Handle mice for glucose metabolism studies (Glucose, insulin tolerance test, glucose stimulated insulin secretion), b. Perform data acquisition and quantitation of mouse experiments (weighing, glucose and serum in vivo) c. Perform islet isolation from mice (will be trained). d. Perform immunohistochemistry in pancreas or placenta f. Maintain established mouse database with assistance from Researcher 1 personnel.
4) Laboratory Management (10%)
a. Maintain the established laboratory equipment and supplies b. Maintain Catalog of vectors, constructs, cell lines, and samples c. Prepare media and reagents/buffers d. Assist Principle Investigator with personnel, and supervising undergraduates f. Present relevant scientific journal articles to lab group for discussion and incorporation of new ideas and procedures to the laboratory protocols.
REQUIRED: (must be on application or resume):
-BS/BA degree with two years of experience or a combination of education and work experience to equal 6 years. -Experience with DNA Binding Assays and ChIP. -Inquisitive, motivated, organized (able to recognize and prioritize work) and responsible. -Work independently and willing to assist others in the lab -Willing to work extensively with animals
-BS/BA or MA/MS in biological sciences, biochemistry, molecular biology, or related discipline is preferred plus 2 years of research experience.
The University of Minnesota, founded in the belief that all people are enriched by understanding, is dedicated to the advancement of learning and the search for truth; to the sharing of this knowledge through education for a diverse community; and to the application of this knowledge to benefit the people of the state, the nation, and the world.