The mission of the Wyss Institute for Biologically Inspired Engineering at Harvard University is to transform human healthcare and the environment by emulating the way nature builds. Developed as an alliance between Harvard and other premier academic and clinical partner institutions, Institute faculty and staff collaborate in high-risk, fundamental research and science-driven technology development. A major focus of the Institute is to translate the technologies developed by its faculty and staff into commercial products and therapies through collaborations with clinical investigators and establishment of corporate alliances. For more information, visit: http://wyss.harvard.edu/.
The Wyss Institute is seeking a highly motivated and innovative Scientist with significant expertise in Tissue Engineering to join a dynamic team dedicated to the discovery, development and translation of novel technologies in cell biology, tissue fabrication, and organ regeneration. The Scientist will lead ongoing initiatives towards the goal of whole organ bioengineering at the Wyss Institute. These studies entail primary and stem cell culture, tissue and organ assembly, maintenance and characterization of organs during maturation in bioreactors, and in vivo implantation. Additional analytical responsibilities include light and fluorescence microscopy, histologic analysis, molecular biology, biochemical assays, and tissue-level physiology. Specifically, this role will be responsible for driving translational approaches to investigate and support our 3D Organ Engineering focus area. Candidates must be willing to spend a majority of time at the bench doing experiments that advance this initiative.
The position reports to the Institute's Operations Director. Under the technical supervision of Organ Engineering Faculty Leads (Christopher Chen and Jennifer Lewis), the Staff Scientist will carry out a broad range of research activities and procedures with some latitude to determine techniques and to establish protocols. The Staff Scientist will perform lab procedures with speed, precision, and understanding of the theory behind these techniques and assays.
Duties and Responsibilities continued in Additional Information
Duties and Responsibilities Continued
PRINCIPAL DUTIES AND RESPONSIBILITIES:
Perform isolation, expansion, and banking of primary cell populations from tissues, iPSC reprogramming and characterization. Utilize experience with cell culture and aseptic technique. Assist in the development of large-scale organ scaffolds working with a multi-disciplinary team that would include technical, clinical, and development collaborators. Monitor and analyze the regenerating organs during ex vivo biomimetic culture, including the collection and analysis of pre-expanded cell populations, organ scaffold preparation and handling, and changing of cell culture media in the bioreactor as needed. Design and conduct implantation experiments in appropriate animal models. The candidate will be responsible for managing scientific and technical activities across this effort, including interactions with numerous other teams.
Independently develop experimental plans and successfully execute research timelines to meet translation goals and milestones.
Establish primary and stem cell-based culture systems.
Implement quantitative in vitro assays for evaluating tissue functionality, stability, and safety.
Provide in-depth expertise and hands-on experience in developing functional assays.
Provide relevant skills include proficiency in cell biology and immunological techniques, including multi-color flow cytometry, immunocytochemistry, ELISA cytotoxicity assays, RNASEQ and cytokine analysis.
Develop and standardize animal protocols, tissue constructs, and surgical procedures for in vivo studies
Participate in writing research proposals, reports, patent applications, presentations and publications, providing critical data, figures or information for grant and patent submissions.
Additional Information: This is a term position for two years with the possibility of renewal.
Salary Grade: 058
Union: 00 - Non Union, Exempt or Temporary
Ph.D. degree in a biological science, bioengineering, or related discipline, with a good understanding of Cellular & Molecular Biology. 3 or more years industry and/or academic experience in biofabrication, tissue engineering, cell biology or related areas. Industry experience required.
Demonstrated broad theoretical and practical knowledge of the fields of biofabrication, tissue engineering, and cell biology Strong scientific track-record demonstrating well organized design and execution of studies Extensive experience with in vivo and in vitro tissue fabrication, characterization, and implantation Demonstrated success in leading and working in cross-functional teams Comfortable in a fast-paced translational environment and able to adjust workload based upon changing priorities Flexible and cooperative spirit with strong commitment to team efforts Strong oral, written, and presentation skills
EQUAL OPPORTUNITY EMPLOYER: We are an equal opportunity employer and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability status, protected veteran status, or any other characteristic protected by law.
Harvard University is devoted to excellence in teaching, learning, and research, and to developing leaders in many disciplines who make a difference globally. The University, which is based in Cambridge and Boston, Massachusetts, has an enrollment of over 20,000 degree candidates, including undergraduate, graduate, and professional students. Harvard has more than 360,000 alumni around the world. T...he University has twelve degree-granting Schools in addition to the Radcliffe Institute for Advanced Study, offering a truly global education. Established in 1636, Harvard is the oldest institution of higher education in the United States.