About the Job We are seeking a highly self-motivated individual with good attitude for a Researcher 1 position to join our group studying polycystic kidney disease. This is a laboratory-based position focuses on transcriptional regulation of the polycystic kidney disease in mouse models. This individual will work closely with a senior member of the laboratory and assist experiments in research projects.
Duties/Responsibilities: This individual will provide biological expertise and techniques in mouse models, tissue culture, microscopy, molecular biology and gene editing.
75% Research Projects 1. Conduct a breeding program of transgenic mice and assist animal experiments:
Log keeping and tracking of mice using tags and ear punches and perform PCR to identify genotypes
Ability to handle mice, knowledge of breeding patterns, and familiarity with Institutional Animal Care and Usage Committee (IACUC) and Research Animal Resources (RAR) regulations
Prepare harvested organs and tissues for biochemical and histological analysis
2. Tissue Culture:
Maintenance of cell lines and preparation of tissue culture reagents
Seeding of experimental plates for plasmid transfection and lentivirus transduction
Harvest cell lysates for DNA, RNA and protein analysis
3. Molecular Biology
IHC, In situ, Molecular cloning, PCR, qRT-PCR, plasmid extraction and DNA sequencing
4. Gene editing
Use CRISPR/Cas9 for genome engineering of cell lines: construction of CRISPR/Cas9 gene targeting reagents, selection of edited cell lines by single cell cloning, PCR and DNA sequencing for validation of edited cell lines.
5. Conduct assigned research experiments by PI
25% Data Analysis
Prepare written and graphical descriptions of results to discuss with PIs
Review scientific literature and technical documents
Collaborate with faculty and post docs in the lab regarding problem solving and data analysis.
Present routine summary reports on research protocols to faculty and post docs.
Troubleshoot methods and techniques for potential improved outcomes.
All required qualifications must be documented on application materials.
Bachelor's degree in biology or a related field of science or a combination of related education and work experience to equal four years.
Documented course work or experience in cell biology, genetics, biochemistry, and molecular biology.
Experience in molecular biology techniques, PCR, cell culture, mouse husbandry, sectioning for IF, IHC and ISH, Western blotting.
Preference will be given to candidate with strong interest in molecular biology and gene editing.
The University of Minnesota, founded in the belief that all people are enriched by understanding, is dedicated to the advancement of learning and the search for truth; to the sharing of this knowledge through education for a diverse community; and to the application of this knowledge to benefit the people of the state, the nation, and the world.