Job Summary: The Research Technician in David Baltimore's lab will investigate the role of T-cell receptors in cancer immunotherapy toward acute myelogenous leukemia (AML) using novel sequencing technologies. This position involves molecular biology, cell biology, and ideally some computational biology. The primary responsibility is to conduct experiments with the guidance of the postdoctoral fellow, but data analysis and meticulous documentation of research are also central to the role.
Research has a large molecular biology component. With the help of the supervisor, technician will PCR clone inserts into vectors, make TCR vectors and inserts, and make Illumina libraries.
Research will involve cell culture by technician. To test the vectors designed above, transfection and electroporation will be used to incorporate vectors. Flow cytometry will be used to analyze fluorescent reporters, and RNA will be isolated from cells by the technician and analyzed using RT-qPCR.
Research will also involve perturbing immune cells, in cell culture and in animal studies, under the guidance of a postdoctoral supervisor. This role will involve euthanizing mice, harvesting tissues, and retroorbital bleeding of mice.
Performs other lab duties such as preparing stock solutions, assisting with lab inventory, and other lab maintenance.
Bachelors degree in biology, biochemistry, or related.
At least one year of experience in biological research (as an undergrad in a lab, over summers, or after).
Must be confident with performing and troubleshooting: PCR, running agarose gels, restriction digest, minipreps, mammalian cell culture technique, use of multichannel pipets, RNA isolation/RT/qPCR.
Must possess exceptional organizational skills and be able to apply these when carrying out experiments and collecting data.
Must be highly motivated and productive, with the ability to both work as part of a team as well as carry out research tasks independently.
Must be able to multitask to maximize productivity.
Experience on experimental techniques that would be of exceptional value: some experience with analysis of high throughput sequencing data is ideal but not required, RNA isolation/RT/qPCR, Next Generation Sequencing (Illumina) Library Preparation.
Experience on any of the following skills would be of great value: CRISPR/CAS design, mammalian cell transfection and electroporation, mouse handling and experimentation (injections, bleeding, euthanizing, harvesting tissues).
At least two years of relevant work experience preferred.
To be considered for this position please visit our web site and apply on line at the following link: jobs.caltech.edu
We are an equal opportunity employer and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability status, protected veteran status, or any other characteristic protected by law.
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